Re: [ccp4bb] Co-expression plasmids

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Mensur Dlakic mdlakic {- at -} MONTANA {- dot -} EDU
Date: 2008-04-02

Next message:
Subject: Re: Co-expression plasmids
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-04-02

Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02

Hi,

If you are expressing just two proteins, you could try a single pET vector with a pCDF vector. The only reason I'm suggesting this is that I had trouble with pET-Duet, but doing each one separately worked first time (plasmid size issue?). I used pET24-a and pCDF-1b - so I had one construct untagged, and the other with a cleavable His-tag.

Cheers

AGS

Date: Wed, 2 Apr 2008 15:55:27 +0200
From: VANRAAIJ@USC.ES
Subject: [ccp4bb] Co-expression plasmids
To: CCP4BB@JISCMAIL.AC.UK

Dear All,Anyone have experience with the NovaGen Duet co-expression vectors? Or can recommend others?
http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
Greetings,Mark Mark J. van RaaijDpto de Bioquímica, Facultad de FarmaciaUniversidad de Santiago15782 Santiago de CompostelaSpainhttp://web.usc.es/~vanraaij/

_________________________________________________________________
Get in touch in an instant. Get Windows Live Messenger now.
http://www.windowslive.com/messenger/overview.html?ocid=TXT_TAGLM_WL_Refresh_getintouch_042008

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Mensur Dlakic mdlakic {- at -} MONTANA {- dot -} EDU
Date: 2008-04-02

Next message:
Subject: Re: Co-expression plasmids
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-04-02