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Re: [ccp4bb] Co-expression plasmids
 

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CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008
Previous message:
Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02 		Next message:
Subject: Re: Co-expression plasmids
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-04-02


Subject: Re: Co-expression plasmids
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-04-02
We have had good experience with the awfully simple minded approach
of using two pET vectors with different antibiotic resistance.
Its the easiest thing to do, and it often works ...

Apologies for the shameless plugin, since there are many good papers
on the subject, but you can read some hints and case studies at:

http://scripts.iucr.org/cgi-bin/paper?S0907444906031003

(its Open Access)

A.


On 2 Apr 2008, at 19:39, P Hubbard wrote:

> Hi,
>
> If you are expressing just two proteins, you could try a single pET
> vector with a pCDF vector. The only reason I'm suggesting this is
> that I had trouble with pET-Duet, but doing each one separately
> worked first time (plasmid size issue?). I used pET24-a and pCDF-1b
> - so I had one construct untagged, and the other with a cleavable
> His-tag.
>
> Cheers
>
> AGS
>
> Date: Wed, 2 Apr 2008 15:55:27 +0200
> From: VANRAAIJ@USC.ES
> Subject: [ccp4bb] Co-expression plasmids
> To: CCP4BB@JISCMAIL.AC.UK
>
> Dear All,
>
> Anyone have experience with the NovaGen Duet co-expression vectors?
> Or can recommend others?
> http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
>
> Greetings,
>
> Mark
>
> Mark J. van Raaij
> Dpto de Bioquímica, Facultad de Farmacia
> Universidad de Santiago
> 15782 Santiago de Compostela
> Spain
> http://web.usc.es/~vanraaij/
>
>
>
>
>
> Get in touch in an instant. Get Windows Live Messenger now.


CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008
Previous message:
Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02 		Next message:
Subject: Re: Co-expression plasmids
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-04-02


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