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Re: [ccp4bb] Co-expression plasmids
 

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CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008
Previous message:
Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02 		Next message:
Subject: 3D model in glass
From: rcoulombe {- at -} LAV {- dot -} BOEHRINGER-INGELHEIM {- dot -} COM rcoulombe {- at -} LAV {- dot -} BOEHRINGER-INGELHEIM {- dot -} COM
Date: 2008-04-02


Subject: Re: Co-expression plasmids
From: Brian Mark brian_mark {- at -} UMANITOBA {- dot -} CA
Date: 2008-04-02
Hi Mark,

Proper co-expression in trans requires that you use plasmids each with
different origins of replication and antibiotic markers, such as p15 +
Kan and ColE1 + Amp. I think the pET vectors of the DUET system use
these two origins (p15 and ColE1). This ensures much more efficient
dual transformation and proper plasmid stability over time. The two
backbone constructions above are used in a variety of pET vectors, so
if you look for this detail, you can co-transform and co-express from
many available plasmids and customize as you like. Suppliers will
tell you the origins that are used in each of the plasmids they sell.

Cheers,

Brian

=========================
Brian L. Mark, MSc, PhD
Assistant Professor
Department of Microbiology
Room 418, Buller Building
University of Manitoba
Winnipeg, Manitoba
CANADA R3T 2N2

Phone (204) 480-1430
Fax (204) 474-7603
Web: http://www.umanitoba.ca/science/microbiology/staff/mark/


On 2-Apr-08, at 8:55 AM, Mark J. van Raaij wrote:

> Dear All,
>
> Anyone have experience with the NovaGen Duet co-expression vectors?
> Or can recommend others?
> http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
>
> Greetings,
>
> Mark
>
> Mark J. van Raaij
> Dpto de Bioquímica, Facultad de Farmacia
> Universidad de Santiago
> 15782 Santiago de Compostela
> Spain
> http://web.usc.es/~vanraaij/
>
>
>
>





CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008
Previous message:
Subject: Re: Co-expression plasmids
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-02 		Next message:
Subject: 3D model in glass
From: rcoulombe {- at -} LAV {- dot -} BOEHRINGER-INGELHEIM {- dot -} COM rcoulombe {- at -} LAV {- dot -} BOEHRINGER-INGELHEIM {- dot -} COM
Date: 2008-04-02


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