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[ccp4bb] Off topic: PDI overexpression in HEK293 cells
 

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CCP4bb <-- 2008 <-- April 2008 <-- 03 April 2008
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From: "Mueller, Juergen-Joachim" jjm {- at -} MDC-BERLIN {- dot -} DE
Date: 2008-04-03 		Next message:
Subject: Re: Off topic: PDI overexpression in HEK293 cells
From: VADIM A KLENCHIN klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-04-03


Subject: Off topic: PDI overexpression in HEK293 cells
From: Kenneth Verstraete kenneth {- dot -} verstraete {- at -} UGENT {- dot -} BE
Date: 2008-04-03
Dear all,

sorry for this off topic question. I am producing the ectodomain of a
human receptor in HEK293T cells by transient expression. The expression
levels are quite low. The protein contains a high number of disulfide
bridges. It has been reported that overexpression of protein disulfide
isomerase can enhance the secretion of proteins with a high disulfide
content. I would like to test the effect of PDI by cotransfecting a
PDI-encoding vector with my expression plasmid. I wonder if anybody has
some experience with PDI-overexpression and if anybody could provide me
an mammalian expression construct for PDI?

Any suggestions are appreciated,

Kenneth Verstraete

Unit for Structural Biology and Biophysics
Laboratory for Protein Biochemistry and Biomolecular Engineering
Ghent University
K.L. Ledeganckstraat 35
9000 Ghent, BELGIUM
Phone: +32-(0)9-264.51.26
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