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Re: [ccp4bb] Off topic: PDI overexpression in HEK293 cells
 

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CCP4bb <-- 2008 <-- April 2008 <-- 03 April 2008
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Subject: Re: Off topic: PDI overexpression in HEK293 cells
From: Kenneth Verstraete kenneth {- dot -} verstraete {- at -} UGENT {- dot -} BE
Date: 2008-04-03 		Next message:
Subject: Re: Your opinion about the attached diffraction pattern
From: Ibrahim Moustafa I {- dot -} moustafa {- at -} PSU {- dot -} EDU
Date: 2008-04-03


Subject: Re: Off topic: PDI overexpression in HEK293 cells
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-04-03
>>With regard to low levels of expression: Is there a particular reason
>>to use 293 cells? Transient expression in Cos-1/Cos-7 cells gives
>>~ 10 fold higher levels of expression because of the plasmid amplification
>>in these cells. (The only requirement is that the plasmid should
>>have SV40 ori; most mammalian vectors do).
>
>HEK293T cells are reported to have high expression levels, especially
>for secretted proteins (Aricescu et al., ACTA D, 2006).

Ah, yes, indeed. Like Cos cells, they do express large SV40 antigen,
so the transient expression levels will be high. I did not know about
it, thinking of "normal" 293 fibroblast line.

Dima

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CCP4bb <-- 2008 <-- April 2008 <-- 03 April 2008
Previous message:
Subject: Re: Off topic: PDI overexpression in HEK293 cells
From: Kenneth Verstraete kenneth {- dot -} verstraete {- at -} UGENT {- dot -} BE
Date: 2008-04-03 		Next message:
Subject: Re: Your opinion about the attached diffraction pattern
From: Ibrahim Moustafa I {- dot -} moustafa {- at -} PSU {- dot -} EDU
Date: 2008-04-03


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