[ccp4bb] off topic - C-ter cleavable his tag

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Off topic: McDonalds Happy Meal and Crystallography
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-04

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Subject: Re: off topic - C-ter cleavable his tag
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-04-04

Subject: off topic - C-ter cleavable his tag
From: Vijay Kumar vijay85 {- at -} GMAIL {- dot -} COM
Date: 2008-04-04

Hi,

I am looking for a vector with a cleavable C-terminal his tag for
prokaryotic protein expression. I found pET-52b but it has also got a
N-terminal cleavable strep-tag which I cannot avoid.

Thanks

Vijay

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Off topic: McDonalds Happy Meal and Crystallography
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-04-04

Next message:
Subject: Re: off topic - C-ter cleavable his tag
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-04-04