[ccp4bb] Off-topic: fungus contamination of SF9 cells

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From: "Randy J {- dot -} Read" rjr27 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2008-04-04

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Subject: off-topic: fungus contamination in SF9 cells (picture attached)
From: Filipp Frank filipp {- dot -} frank {- at -} MAIL {- dot -} MCGILL {- dot -} CA
Date: 2008-04-04

Subject: Off-topic: fungus contamination of SF9 cells
From: Filipp Frank filipp {- dot -} frank {- at -} MAIL {- dot -} MCGILL {- dot -} CA
Date: 2008-04-04

Dear all,

sorry for this off topic question. I am using baculovirus/insect cell
expression and I have had persisting problems with contamination that
seems to be some kind of fungus. I have a picture attached showing the
filamentous structure of it. This contamination has persisted for a
few months now and always seems to occur when we go to large 2.8 L
flasks (however, there was also one instant where this was not the
case). We have autoclaved all flasks for 90 minutes each and still we
get contamination.

Does anybody have experience with this kind of contamination? And what
would you suggest for us to do?
We will (again) disinfect our incubator and the hood. Also we are
thinking about using an antimycotic/antifungal drug to get rid of this
problem.

Thanks a lot for any suggestion!

Filipp

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Next message:
Subject: off-topic: fungus contamination in SF9 cells (picture attached)
From: Filipp Frank filipp {- dot -} frank {- at -} MAIL {- dot -} MCGILL {- dot -} CA
Date: 2008-04-04