Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Low resol structure
From: Jacqui Gulbis jgulbis {- at -} WEHI {- dot -} EDU {- dot -} AU
Date: 2008-04-14

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Subject: Re: Fwd: crystallisation robot
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-04-14

Subject: Re: Fwd: crystallisation robot
From: Janet Newman Janet {- dot -} Newman {- at -} CSIRO {- dot -} AU
Date: 2008-04-14

We have both a Phoenix and a Mosquito - for one-subwell 96 well plate
both are under 3 minutes from the time you put the first droplet down
until you have to seal the plate. The actual protocols take longer
(particularly on the Phoenix) as you have to wash, transfer reservoir
etc.

We manage to fit in all our grief into that time period, so that we
don't add any exposure to the droplets, but get in world-class hand
wringing anyways.

Janet

_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
JOE CRYSTAL
Sent: Tuesday, 15 April 2008 7:10 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Hi,

Does anyone have information about how long it takes to set up a 96-well
tray for the crystallization robots available? Besides cost per tray
and maintenance cost, another important feature we consider is the time
for setting up a 96-well tray. It is an important factor since we are
talking about sub-microliter drops.

Best,

Joe

On Fri, Jan 18, 2008 at 12:28 PM, Lisa A Nagy wrote:

Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.

Lisa

--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
nagy@uab.edu

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of

Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK

Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein in the - technical term - teeny drop to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though the oil ,
and you'd like to slow up equilibration. of course (2) slows up the
robotics a little, but both should be trivial to set up..

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Low resol structure
From: Jacqui Gulbis jgulbis {- at -} WEHI {- dot -} EDU {- dot -} AU
Date: 2008-04-14

Next message:
Subject: Re: Fwd: crystallisation robot
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-04-14