Re: [ccp4bb] Optimization of needle crystals?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Optimization of needle crystals?
From: Hubing Lou hl254 {- at -} ST-ANDREWS {- dot -} AC {- dot -} UK
Date: 2008-04-16

Next message:
Subject: Re: Optimization of needle crystals?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2008-04-16

Subject: Re: Optimization of needle crystals?
From: Jon Wright wright {- at -} ESRF {- dot -} FR
Date: 2008-04-16

You may be able to check whether the cyrstals are protein or detergent
this using powder diffraction. (please excuse the shameless plug):

Acta Cryst. (2008). A64, 169-180 "Powder crystallography on macromolecules"
I. Margiolaki and J. P. Wright
http://journals.iucr.org/a/issues/2008/01/00/sc5011/index.html

Good luck,

Jon

Hubing Lou wrote:
> You didn't mention which detergent in the conditions. From the picture it looks
> like crystals of detergent.
>

> Quoting Ngo Duc Tri :
>> > Dear ccp4 users,
>> > I crystallized a membrane protein and got some conditions showing the small
>> > needle crystals. All condition contained Mg2+ and high buffer pH.
>> > I learn that it is difficult to optimize the needle crystal. However I
>> > would like to ask your experience how to optimize in this case.
>> > Here is the attached picture of my crystal. Thank you very much for your
>> > advices!
>> >
>> > My best regards,
>> > TriNgo
>> > PhD Student - Sungkyunkwan University, Korea
>> >

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Optimization of needle crystals?
From: Hubing Lou hl254 {- at -} ST-ANDREWS {- dot -} AC {- dot -} UK
Date: 2008-04-16

Next message:
Subject: Re: Optimization of needle crystals?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2008-04-16