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Re: [ccp4bb] How to dissolve a hydrophobic peptide? |
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CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: How to dissolve a hydrophobic peptide? From: David Briggs drdavidcbriggs {- at -} GMAIL {- dot -} COM Date: 2008-04-17 This might help... Hassell AM, An G, Bledsoe RK, Bynum JM, Carter HL 3rd, Deng SJ, Gampe RT, Grisard TE, Madauss KP, Nolte RT, Rocque WJ, Wang L, Weaver KL, Williams SP, Wisely GB, Xu R, Shewchuk LM. Abstract Crystallization of protein-ligand complexes. Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):72-9. Epub 2006 Dec 13. Review. PMID: 17164529 [PubMed - indexed for MEDLINE] hth Dave On 16/04/2008, Jiamu Du > Dear All, > I want to crystallize a mAb Fab in complex with a hydrophobic cyclic > peptide. The peptide contains 1 hydrophilic residue, 2 Cys, and 9 > hydrophobic residues. The problem is that I can not dissolve this peptide in > common buffers. I think it is need to dissolve it in some organic solvent, > such as DMSO. But organic solvent is harmful to protein. > Is there any sugestions for this situation? > > Thanks. > > -- > Jiamu Du > State Key Laboratory of Molecular Biology > Institute of Biochemistry and Cell Biology Shanghai Institutes for > Biological Sciences > Chinese Academy of Sciences (CAS) -- ============================ David C. Briggs PhD Father & Crystallographer http://www.dbriggs.talktalk.net AIM ID: dbassophile ============================ CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
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