Re: [ccp4bb] question on how crystals form

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
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Subject: Re: question on how crystals form
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-05-05

Hi Mike,

how does your protein look on a sizing column ? Perhaps a dimer ? That
would then explain the tendency to form dimers in different crystal
forms, as it is a biological relevant dimer. for the eventuality that
you have no clue how the sizing looks like, run one and find out.

Juergen

Michael Colaneri wrote:

> Dear colleagues,
>
> I have a structure with two identical dimers per asymmetric unit. If
> the dimers are identical in different crystalline environments and in
> different crystal forms they should be particularly stable.
>
> How does such a stable dimer crystallize? Is it necessay to pre-exist
> in the specific conformation in solution (even to a lesser extent than
> a major monomeric form) or a very stable dimer can assemble from the
> very beginning durnig crystallization? And can the same dimer form
> during crystallization in different crystalline environments without
> it being present in solution to any (even infinitesimal) extent?
>
> I would appreciate all responses.
>
> Thanks.
>
> Mike Colaneri

--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: HKL2000 and gcc4
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-05-05

Next message:
Subject: Re: Negative density around C of COO-
From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2008-05-05