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Re: [ccp4bb] negative LLG in Phaser run
 

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CCP4bb <-- 2008 <-- May 2008 <-- 06 May 2008
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Subject: Deadline EMBO Practical Course on X-ray crystal structure determination of macromolecules
From: Rob Meijers ccp4spamalot {- at -} YAHOO {- dot -} COM
Date: 2008-05-06 		Next message:
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Subject: Re: negative LLG in Phaser run
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-05-06
Dear Oganesyan,
That means the second component is not right based on the number of LLG
and PACK.
Please see that for more details.
http://www-structmed.cimr.cam.ac.uk/phaser/faq.html
You should check that model and redo MR again.
Good luck!
leo
Oganesyan, Vaheh wrote:
>
> Phaser experts,
>
> When running Phaser I see LL gain becoming negative closer to the end.
> What would that mean?
>
> Resolution ~3.0 A, s.g. I222, 2 complexes per a.u.
>
> Here is the line from log file:
>
> ANNOTATION: RFZ=7.6 TFZ=8.9 PAK=0 LLG=142 RFZ=6.6 TFZ=18.0 PAK=0
> LLG=150 RFZ=4.6
>
> TFZ=12.8 PAK=0 LLG=-44 RFZ=3.8 TFZ=5.8 PAK=19 LLG=-908
>
> Thank you in advance.
>
> ///______________________/
>
> /Oganesyan Vaheh, Ph.D/
> /Antibody Discovery/
> /MedImmune, Inc./
>
>
>
>
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CCP4bb <-- 2008 <-- May 2008 <-- 06 May 2008
Previous message:
Subject: Deadline EMBO Practical Course on X-ray crystal structure determination of macromolecules
From: Rob Meijers ccp4spamalot {- at -} YAHOO {- dot -} COM
Date: 2008-05-06 		Next message:
Subject: optimization of needle custers
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-05-06


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