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Re: [ccp4bb] Negative density around C of COO- |
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CCP4bb navigationCCP4bb <-- 2008 <-- May 2008 <-- 06 May 2008Subject: Re: Negative density around C of COO- From: Wim Burmeister wpb {- at -} EMBL-GRENOBLE {- dot -} FR Date: 2008-05-06 > Dear all: > I am noticing that in some of my structures, at 1.5 A resolution, 1) > there is some negative density around the C of the carboxyl groups. > 2) I also notice the negative density around S in a disulfide region. > It is as though the disulfide is broken. > > Could some body enlighten me on this feature? > > > The other original reference for the radiation damage effect on carboxyls is actually: Burmeister, W. P. Structural changes in cryo-cooled protein crystals owed to radiation damage. Acta Cryst. D 56, 328-341 (2000). Greetings Wim Burmeister -- ******************************************************************************* Wim Burmeister Professeur, Membre de l'Institut Universitaire de France Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS 6 rue Jules Horowitz B.P. 181, F-38042 Grenoble Cedex 9 FRANCE E-mail: wpb@embl-grenoble.fr Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 94 00 http://www2.ujf-grenoble.fr/pharmacie/laboratoires/gdrviro ******************************************************************************* CCP4bb navigationCCP4bb <-- 2008 <-- May 2008 <-- 06 May 2008 |
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