Re: [ccp4bb] Negative density around C of COO-

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Negative density around C of COO-
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-05-06

Subject: Re: Negative density around C of COO-
From: Wim Burmeister wpb {- at -} EMBL-GRENOBLE {- dot -} FR
Date: 2008-05-06

Narayanan Ramasubbu a écrit :
> Dear all:
> I am noticing that in some of my structures, at 1.5 A resolution, 1)
> there is some negative density around the C of the carboxyl groups.
> 2) I also notice the negative density around S in a disulfide region.
> It is as though the disulfide is broken.
>
> Could some body enlighten me on this feature?
>
>
>

The other original reference for the radiation damage effect on carboxyls
is actually:

Burmeister, W. P.
Structural changes in cryo-cooled protein crystals owed to radiation damage.
Acta Cryst. D 56, 328-341 (2000).

Greetings

Wim Burmeister

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: optimization of needle custers
From: amit sharma 3112amit {- at -} GMAIL {- dot -} COM
Date: 2008-05-06

Next message:
Subject: Re: Negative density around C of COO-
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-05-06