[ccp4bb] phasing at low resolution

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CCP4bb <-- 2008 <-- May 2008 <-- 09 May 2008

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Subject: Re: How to generate two molecules of proteins and two chains of RNA in CNS 1.2
From: Stuart Endo-Streeter stuart {- dot -} endostreeter {- at -} DUKE {- dot -} EDU
Date: 2008-05-09

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Subject: Re: phasing at low resolution
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-05-09

Subject: phasing at low resolution
From: sajid akthar b_sajid_ali {- at -} YAHOO {- dot -} CO {- dot -} IN
Date: 2008-05-09

Hi All
I have a data set of Se-Met crystal 3.65A resolution collected at Inflection and peak wavelength. Also I have a data set at same resolution in native form. The protein size is 28000 dalton. I dont have any model for this protein. So I started to find the heavy atom peak search to find out "Se" positions using Shelx programe. From ShelxD and E, I got refined 12 Se sites with two molecules per AU. This prediction is consistent with the expected number of Se incorporation per molecule.
Since I dont have any model for my protein, I am not able to go beyond this. Though I'm trying to reproduce good diffracting crystals, they diffracts at this level only. Some native crystals diffaracting upto 3.29A. But they are twinned data set. So it is tough to process those data set.
Can I do phasing for the model with the present 12 sites. Please give me your suggestions for this problem.
Thank you
Sajid

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CCP4bb <-- 2008 <-- May 2008 <-- 09 May 2008

Next message:
Subject: Re: phasing at low resolution
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-05-09