Re: [ccp4bb] Two off-topic questions

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2008 <-- May 2008 <-- 12 May 2008

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Subject: Re: Two off-topic questions
From: Hidong Kim HKim {- at -} DECODE {- dot -} COM
Date: 2008-05-12

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Subject: Re: Two off-topic questions
From: "Yong, Wei" WYong {- at -} MCW {- dot -} EDU
Date: 2008-05-12

Subject: Re: Two off-topic questions
From: Martin Hallberg Martin {- dot -} Hallberg {- at -} KI {- dot -} SE
Date: 2008-05-12

Hi Wei Yong,

On May 12, 2008, at 4:12 PM, Yong, Wei wrote:
>
> I have solved a pig mitochondrial protein crystal structure whose
> molecular weight is about 95kd. However, I do not have the sequence
> information of this protein. It is weird that I could not blast its
> sequence in pig genome. Because pig sequences of known proteins are
> very similar to their human homologous, I used this protein's human
> homologous sequence to fit into electron density. Currently, I am
> planning to sequence the full length sequence of the protein.
> However, I was not able to successfully extract mRNA from pig liver
> using Qiagen RNeasy kit (I only tried once). Does any body have
> ideas about how to extract mRNA from pig live (or animal tissue in
> general)? Are there any other database I can search for pig protein
> sequence information?

You can use the Copenhagen PigEST database.
http://pigest.ku.dk/server/index.html

Cheers,

Martin

.
B. Martin Hallberg, PhD
Assistant professor
Department of Cell and Molecular Biology
Medical Nobel Institute
Karolinska Institutet
Nobels väg 3
SE-171 77 Stockholm
Sweden

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CCP4bb <-- 2008 <-- May 2008 <-- 12 May 2008

Previous message:
Subject: Re: Two off-topic questions
From: Hidong Kim HKim {- at -} DECODE {- dot -} COM
Date: 2008-05-12

Next message:
Subject: Re: Two off-topic questions
From: "Yong, Wei" WYong {- at -} MCW {- dot -} EDU
Date: 2008-05-12