Re: [ccp4bb] Two off-topic questions

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: HKL2000 zoom window
From: Eric Salgado esalgado {- at -} POPMAIL {- dot -} UCSD {- dot -} EDU
Date: 2008-05-12

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Subject: Re: Two off-topic questions
From: "Scapin, Giovanna" giovanna_scapin {- at -} MERCK {- dot -} COM
Date: 2008-05-12

Subject: Re: Two off-topic questions
From: Partha Chakrabarti ppchak {- at -} GMAIL {- dot -} COM
Date: 2008-05-12

Or, make sure that you don't post it on Monday, some people, sometimes
are in very bad mood on Mondays, I am not going to explain why.. :P

On Mon, May 12, 2008 at 5:57 PM, Yong, Wei wrote:
>
> Sorry that I missed a letter. I wanted to extract mRNA from pig liver. Thank you, Frank, for having taught me a lesson. I will carefully check my emails before I send them to ccp4bb.
>
> I am looking forward to getting suggestions.
> Best wishes
> Wei Yong
>
>
> ________________________________
>
> From: Frank von Delft [mailto:frank.vondelft@sgc.ox.ac.uk]
> Sent: Mon 5/12/2008 11:12 AM
> To: Yong, Wei
> Cc: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Two off-topic questions
>
>
>
>
>> only tried once). Does any body have ideas about how to extract
mRNA from pig live (or animal tissue in general)?
>>
> From pig live? Yeah sure, toss it in a blender -- you can tell whether
> it's alive by the squealing. You may need quite a large blender,
> though, and a crane to lift it, pigs are deceptively large.
>
> It's trickier to keep the pig alive even after extracting the mRNA. The
> subject was dealt with at some length as far back as the late 16th
> century by a well-known English dramatist, although he framed the
> problem in more general terms. But do you really need a pound of the stuff?
>
> phx.
>

--
MRC National Institute for Medical Research
Division of Molecular Structure
The Ridgeway, NW7 1AA, UK
Email: pchakra@nimr.mrc.ac.uk
Phone: + 44 208 816 2515

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: HKL2000 zoom window
From: Eric Salgado esalgado {- at -} POPMAIL {- dot -} UCSD {- dot -} EDU
Date: 2008-05-12

Next message:
Subject: Re: Two off-topic questions
From: "Scapin, Giovanna" giovanna_scapin {- at -} MERCK {- dot -} COM
Date: 2008-05-12