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Re: [ccp4bb] problem of crystallization
 

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CCP4bb <-- 2008 <-- May 2008 <-- 13 May 2008
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Subject: problem of crystallization
From: Jennifer Han-Chun Tsai jhc {- dot -} tsai {- at -} GMAIL {- dot -} COM
Date: 2008-05-13 		Next message:
Subject: PhD Studentship in Structural Biology of Metalloproteins
From: "Hough, MA (Michael)" m {- dot -} a {- dot -} hough {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-05-13


Subject: Re: problem of crystallization
From: Stephen Graham stepheng {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-05-13
Try reductive methylation of surface lysines.

http://dx.doi.org/10.1016/j.str.2006.09.005

Cheers,

Stephen

On 5/13/08, Jennifer Han-Chun Tsai wrote:
> Hi,
>
> This topic is not related to CCP4. I am having problem of crystallizing
> one protein. It's a pretty small protein with size around 15kDa. I have
> stock concentration around 100mg/mL. Crystallization plates I set up are
> with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had been
> set up at least one week. Only around 5 wells per plate or less formed
> precipitation. The rest of wells are pretty clear still. Is there any
> suggestion for reducing protein solubility or increasing the chance of
> getting crystals?
>
> Thanks for your time,
> Jennifer


--
Dr Stephen Graham
Nuffield Medical Fellow
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
Roosevelt Drive
Oxford OX3 7BN
United Kingdom
Phone: +44 1865 287 549
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