Re: [ccp4bb] problem of crystallization

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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008

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Subject: Graphics software to show epitope foot-print
From: "ANSV (Anders Svensson)" ansv {- at -} NOVONORDISK {- dot -} COM
Date: 2008-05-14

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Subject: any review on protein-protein complex crystallization?
From: Maarten Dewilde maarten {- dot -} dewilde {- at -} PHARM {- dot -} KULEUVEN {- dot -} AC {- dot -} BE
Date: 2008-05-14

Subject: Re: problem of crystallization
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-05-14

... your protein is the dream of any NMR spectroscopist. Small and
ultra-soluble.

Maybe just do the structure by NMR? It should be totally
straightforward for any NMR lab.

A.

On May 13, 2008, at 18:16, Jennifer Han-Chun Tsai wrote:

> Hi,
>
> This topic is not related to CCP4. I am having problem of
> crystallizing one protein. It's a pretty small protein with size
> around 15kDa. I have stock concentration around 100mg/mL.
> Crystallization plates I set up are with concentration of 10mg/mL,
> 30mg/mL and 50mg/mL. All the plates had been set up at least one
> week. Only around 5 wells per plate or less formed precipitation.
> The rest of wells are pretty clear still. Is there any suggestion
> for reducing protein solubility or increasing the chance of getting
> crystals?
>
> Thanks for your time,
> Jennifer

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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008

Previous message:
Subject: Graphics software to show epitope foot-print
From: "ANSV (Anders Svensson)" ansv {- at -} NOVONORDISK {- dot -} COM
Date: 2008-05-14