[ccp4bb] any review on protein-protein complex crystallization?

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008

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From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-05-14

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Subject: any review on protein-protein complex crystallization?
From: Maarten Dewilde maarten {- dot -} dewilde {- at -} PHARM {- dot -} KULEUVEN {- dot -} AC {- dot -} BE
Date: 2008-05-14

Dear CCP4bb,

Can anyone point me to good reviews or books which describe how to
crystallize protein-protein complexes (or anyone willing to share his/her
experience)? In particular I'm interested in:

(1) which tests to perform on your complex before you even start thinking
about crystallization

(2) what should you take in account when you start to crystallize (any
other than 'use the Radaev and Sun' screen)

(3) how to increase the success rate of crystallization (cross linking?
co-expression?)

(4) any alternative techniques when crystallization fails (any other than
NMR)

I would be very grateful!

Thanks in advance,

Maarten

__________________________

Maarten Dewilde - PhD student

Laboratory for Pharmaceutical Biology
O&N2 - PB 824 - Herestraat 49
3000 Leuven

Maarten.Dewilde@pharm.kuleuven.be
tel: +32 16 323434
fax: +32 16 323460

Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm

CCP4bb navigation

CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008

Previous message:
Subject: Re: problem of crystallization
From: Anastassis Perrakis a {- dot -} perrakis {- at -} NKI {- dot -} NL
Date: 2008-05-14

Next message:
Subject: Fixing waters around metal atom
From: Jendrek alnyds {- at -} GMAIL {- dot -} COM
Date: 2008-05-14