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Re: [ccp4bb] problem of crystallization
 

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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008
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Subject: Call for synchrotron beam time at EMBL Hamburg
From: Victor Lamzin victor {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2008-05-14 		Next message:
Subject: refinement problem
From: parkash vimal {- dot -} parkash {- at -} HELSINKI {- dot -} FI
Date: 2008-05-14


Subject: Re: problem of crystallization
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2008-05-14
I would suggest to open the wells, add some hefty precipitant
to the reservoir, and close the wells again.
The point is to drive the equilibrium further towards high concentration
by drawing more water into the reservoir.

It therefore does not matter what the supplemental precipitant is.
Concentrated AMS, PEG, whatever as long as it has no volatile
component which might uncontrollably affect pH in the drop.

BR


-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Anastassis Perrakis
Sent: Wednesday, May 14, 2008 1:28 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] problem of crystallization

... your protein is the dream of any NMR spectroscopist. Small and
ultra-soluble.

Maybe just do the structure by NMR? It should be totally straightforward for
any NMR lab.

A.

On May 13, 2008, at 18:16, Jennifer Han-Chun Tsai wrote:

> Hi,
>
> This topic is not related to CCP4. I am having problem of
> crystallizing one protein. It's a pretty small protein with size
> around 15kDa. I have stock concentration around 100mg/mL.
> Crystallization plates I set up are with concentration of 10mg/mL,
> 30mg/mL and 50mg/mL. All the plates had been set up at least one week.
> Only around 5 wells per plate or less formed precipitation.
> The rest of wells are pretty clear still. Is there any suggestion for
> reducing protein solubility or increasing the chance of getting
> crystals?
>
> Thanks for your time,
> Jennifer

CCP4bb navigation

CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008
Previous message:
Subject: Call for synchrotron beam time at EMBL Hamburg
From: Victor Lamzin victor {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2008-05-14 		Next message:
Subject: refinement problem
From: parkash vimal {- dot -} parkash {- at -} HELSINKI {- dot -} FI
Date: 2008-05-14


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