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[ccp4bb] refinement problem
 

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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008
Previous message:
Subject: Re: problem of crystallization
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2008-05-14 		Next message:
Subject: Re: pdb format
From: Nicolas Soler soler {- at -} ICSN {- dot -} CNRS-GIF {- dot -} FR
Date: 2008-05-14


Subject: refinement problem
From: parkash vimal {- dot -} parkash {- at -} HELSINKI {- dot -} FI
Date: 2008-05-14
Hi,
I have one structural refinement problem.
I am working on a protein crystals which diffracted to 2.8 Å. But when I
refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get high
B-factors around 70. But if I do TLS refinement, the R-factors lower
down and B-factors come down to 40.

My question is; Are TLS refined B-factors real?? Can I trust them?
or what else could be done to lower down the B.factors?

BR
vimal

CCP4bb navigation

CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008
Previous message:
Subject: Re: problem of crystallization
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2008-05-14 		Next message:
Subject: Re: pdb format
From: Nicolas Soler soler {- at -} ICSN {- dot -} CNRS-GIF {- dot -} FR
Date: 2008-05-14


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