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Re: [ccp4bb] refinement problem
 

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CCP4bb <-- 2008 <-- May 2008 <-- 14 May 2008
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Subject: Position in protein crystallography, Novartis. Insts. for Biomedical Research - Emeryville
From: "Bussiere, Dirksen" dirksen {- dot -} bussiere {- at -} NOVARTIS {- dot -} COM
Date: 2008-05-14 		Next message:
Subject: Re: any review on protein-protein complex crystallization?
From: Jesse Sundlov jesse {- at -} JHU {- dot -} EDU
Date: 2008-05-14


Subject: Re: refinement problem
From: krish krishna {- dot -} jnu {- at -} GMAIL {- dot -} COM
Date: 2008-05-14
Hi,
It would be nice if you mention more clearly how you have done
TLS..i.e.,domains or individual residues or molecules !! You should keep in
mind that you have 2.8A data. I would do TLSANL with domains or molecules.

Best regards,
Krishna Ch
PhD Student
Hannover Medical school
Germany
On Wed, May 14, 2008 at 11:36 AM, parkash wrote:

> Hi,
> I have one structural refinement problem.
> I am working on a protein crystals which diffracted to 2.8 Å. But when I
> refine through REFMAC5, with 0.1 wt(geometry to x-ray terms), I get high
> B-factors around 70. But if I do TLS refinement, the R-factors lower down
> and B-factors come down to 40.
>
> My question is; Are TLS refined B-factors real?? Can I trust them?
> or what else could be done to lower down the B.factors?
>
> BR
> vimal
>
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