Re: [ccp4bb] any review on protein-protein complex crystallization?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: refinement problem
From: krish krishna {- dot -} jnu {- at -} GMAIL {- dot -} COM
Date: 2008-05-14

Next message:
Subject: UV light source for protein xtal detection
From: Torres-Larios Alfredo torres {- at -} IFC {- dot -} UNAM {- dot -} MX
Date: 2008-05-14

Subject: Re: any review on protein-protein complex crystallization?
From: Jesse Sundlov jesse {- at -} JHU {- dot -} EDU
Date: 2008-05-14

This may be obvious, but I would run a size-exclusion column on the complex
first. This will give you an idea as to how well these two proteins "stick"
together.

On Wed, May 14, 2008 at 4:34 AM, Maarten Dewilde <
maarten.dewilde@pharm.kuleuven.ac.be> wrote:

> Dear CCP4bb,
>
>
>
> Can anyone point me to good reviews or books which describe how to
> crystallize protein-protein complexes (or anyone willing to share his/her
> experience)? In particular I'm interested in:
>
>
>
> (1) which tests to perform on your complex before you even start
> thinking about crystallization
>
> (2) what should you take in account when you start to crystallize (any
> other than 'use the Radaev and Sun' screen)
>
> (3) how to increase the success rate of crystallization (cross linking?
> co-expression?)
>
> (4) any alternative techniques when crystallization fails (any other
> than NMR)
>
>
>
> I would be very grateful!
>
>
>
> Thanks in advance,
>
>
>
> Maarten
>
>
>
>
>
>
>
>
>
> __________________________
>
>
>
> Maarten Dewilde - PhD student
>
>
>
> Laboratory for Pharmaceutical Biology
> O&N2 - PB 824 - Herestraat 49
> 3000 Leuven
>
>
>
> Maarten.Dewilde@pharm.kuleuven.be
> tel: +32 16 323434
> fax: +32 16 323460
>
>
>
>
>
>
> Disclaimer: http://www.kuleuven.be/cwis/email_disclaimer.htm for more
> information.
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: refinement problem
From: krish krishna {- dot -} jnu {- at -} GMAIL {- dot -} COM
Date: 2008-05-14

Next message:
Subject: UV light source for protein xtal detection
From: Torres-Larios Alfredo torres {- at -} IFC {- dot -} UNAM {- dot -} MX
Date: 2008-05-14