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Re: [ccp4bb] UV light source for protein xtal detection
 

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CCP4bb <-- 2008 <-- May 2008 <-- 15 May 2008
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From: Crina Nimigean crn2002 {- at -} MED {- dot -} CORNELL {- dot -} EDU
Date: 2008-05-15 		Next message:
Subject: Re: UV light source for protein xtal detection
From: Mark Agacan M {- dot -} Agacan {- at -} DUNDEE {- dot -} AC {- dot -} UK
Date: 2008-05-15


Subject: Re: UV light source for protein xtal detection
From: Li Zhijie zhijie {- dot -} li {- at -} UTORONTO {- dot -} CA
Date: 2008-05-15
Hello,

You may want to have a look at the UV LEDs, which should be the cheapest
option if you only need a specific wavelenth.

I found this on google: http://www.3dzled.com/other.html. It seems that they
can make 280nM LEDs. It is interesting to note that they also said these
LEDs' "Wavelength tolerance is usually within +/- 5 nm. For example 254 nm
would be 249 nm to 259 nm and 415 nm would be 410 nm to 415 nm or 415 nm to
420 nm" - apparently not as pure as those generated by monochrometers, but
should be good enough for quatitating protein or exciting some fluorophores.
I wonder if the microscope makers would ever consider using these instead of
those multi-thousand $ light sources.

One more thing: do not forget that 280nm UV is extremely harmful to human
eyes and skin.

Zhijie Li

----- Original Message -----
From: "Torres-Larios Alfredo"
To:
Sent: Wednesday, May 14, 2008 7:59 PM
Subject: [ccp4bb] UV light source for protein xtal detection


> Dear all,
>
> Here's another non CCP4 question: does anyone know a cheap alternative to
> set up a UV source at 280 nm? I'd really like to have one :), but I really
> don't have the $20K Dlls needed to buy a UV/white light source from the
> crystallographic vendors :(.
>
> Thanks so much in advance for your answers, Alfredo.
>
> Alfredo Torres-Larios, PhD
> Assistant Professor
> Instituto de Fisiologia Celular, UNAM.
> Ciudad Universitaria, Mexico
>
> ----------------------------------------------------------------
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