[ccp4bb] Additive screens

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: oxidised cys
From: Flip Hoedemaeker flip {- at -} KEYDP {- dot -} COM
Date: 2007-04-12

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Subject: Re: Additive screens
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-12

Subject: Additive screens
From: Brian Mark brian_mark {- at -} UMANITOBA {- dot -} CA
Date: 2007-04-12

Hello everyone,

Does anyone have suggestions or comments on the various additive
screens that are available?

I was looking at the kit from Hampton (HR2-138 Additive Screen HT) in
96-well format. It appears that it should be straight forward to
array out our current protein/mother liquor condition into a 96-plate
and then add in the additives from the deep-well block provided by
the company. The additives come 10x concentrated in a 96-well deep-
well block for dilution into the arrayed crystallization condition.

Does anyone do this routinely for the crystals they grow? It would
be interesting to know how often people find a systematic approach
using such a kit actually improves the quality/size etc. of their
crystals.

Cheers,

Brian

-------------------------------------------------------
Brian L. Mark, MSc, PhD
Department of Microbiology
Room 418, Buller Building
University of Manitoba
Winnipeg, Manitoba
CANADA R3T 2N2

Phone (204) 480-1430
Fax (204) 474-7603
Web: http://www.umanitoba.ca/science/microbiology/staff/mark/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: oxidised cys
From: Flip Hoedemaeker flip {- at -} KEYDP {- dot -} COM
Date: 2007-04-12

Next message:
Subject: Re: Additive screens
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-12