Re: [ccp4bb] Additive screens

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Additive screens
From: Brian Mark brian_mark {- at -} UMANITOBA {- dot -} CA
Date: 2007-04-12

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Date: 2007-04-12

Subject: Re: Additive screens
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-12

Hi,

We use additives extensively. Pretty much all available kits, as well as
some of our own design. My favorite is Nextal (Qiagen) kit named the Salt
Optimizer.

Artem

> Hello everyone,
>
> Does anyone have suggestions or comments on the various additive
> screens that are available?
>
> I was looking at the kit from Hampton (HR2-138 Additive Screen HT) in
> 96-well format. It appears that it should be straight forward to
> array out our current protein/mother liquor condition into a 96-plate
> and then add in the additives from the deep-well block provided by
> the company. The additives come 10x concentrated in a 96-well deep-
> well block for dilution into the arrayed crystallization condition.
>
> Does anyone do this routinely for the crystals they grow? It would
> be interesting to know how often people find a systematic approach
> using such a kit actually improves the quality/size etc. of their
> crystals.
>
> Cheers,
>
> Brian
>
> -------------------------------------------------------
> Brian L. Mark, MSc, PhD
> Department of Microbiology
> Room 418, Buller Building
> University of Manitoba
> Winnipeg, Manitoba
> CANADA R3T 2N2
>
> Phone (204) 480-1430
> Fax (204) 474-7603
> Web: http://www.umanitoba.ca/science/microbiology/staff/mark/

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Additive screens
From: Brian Mark brian_mark {- at -} UMANITOBA {- dot -} CA
Date: 2007-04-12

Next message:
Subject: Job offer: beamline scientists at PETRA III
From: Edgar Weckert edgar {- dot -} weckert {- at -} DESY {- dot -} DE
Date: 2007-04-12