Re: [ccp4bb] crystallisation

- Protein crystallography

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- Protein purification
- Crystallisation

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: "Nadir T {- dot -} Mrabet" Nadir {- dot -} Mrabet {- at -} MEDECINE {- dot -} UHP-NANCY {- dot -} FR
Date: 2008-06-02

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Subject: Re: crystallisation
From: Navdeep Sidhu navdeep {- dot -} sidhu {- at -} USASK {- dot -} CA
Date: 2008-06-02

What is the room temperature diffraction like? If that's good, you might like to
optimize cryo. If I am not mistaken, PEG 3350 has been reported to cryoprotect
at 20-35% with or without 5-10% glycerol. You might also like to try including
some glycerol in the mother liquor--it may better crystal tolerance of glycerol
as cryoprotectant.

Sincerely,
Navdeep

---
Navdeep Sidhu
Graduate Student
University of Saskatchewan

---
Quoting sajid akthar :

>
> Dear All
>
> My protein size is ~30kD and crystallizes with
> 19%Peg3350, 0.2M Nacl, and 0.1M Na Cacodylate buffer.
>
> Please refer the attached crystal image with this. The
> crystal looks like good enough for home source. These
> crystals appears in 4-5 days at room temp.
>
> Sometimes I'm getting crystals like this, but very few
> in 24 well tray. Most of the time, I found the drop
> contains needles. If I reduce the precipitant little
> bit, I wont find any change in the drop even after
> long time. Changing pH (or temp)of the buffer does not
> help me any better. The crystal appears only around
> 5.5pH.
>
> The problem is mosaicity. This crystal diffracted in
> home source upto 3.2A and the mosaicity is 2.5degree.
> Almost all the good crystal like this having same
> mosaicity.
>
> Good cryo condition so far that I found was
> 10%Glycerol with mother liquor. Other conditions
> weekens the diffraction quality or increase mosaicity.
>
> In many crystal I could see some crack in the middle
> of the crystal, it looks like twin crystal. Or the
> crystal appears with some sattelite crystals.
>
> Can anyone suggest me some good way to overcome these
> problems.
>
> Thankz
>
> Sajid
>
>
>
> From Chandigarh to Chennai - find friends all over India. Go to
> http://in.promos.yahoo.com/groups/citygroups/
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: 3D model building without CRT monitor
From: "Nadir T {- dot -} Mrabet" Nadir {- dot -} Mrabet {- at -} MEDECINE {- dot -} UHP-NANCY {- dot -} FR
Date: 2008-06-02

Next message:
Subject: EMBO Workshop Announcement
From: Kim Henrick henrick {- at -} EBI {- dot -} AC {- dot -} UK
Date: 2008-06-02