Re: [ccp4bb] gfp problem

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Subject: gfp problem
From: Cui Sheng sheng {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-06-02

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Subject: Re: gfp problem
From: "Mark J {- dot -} van Raaij" vanraaij {- at -} USC {- dot -} ES
Date: 2008-06-03

Subject: Re: gfp problem
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-06-02

Hi,

It soulds like you have serious disorder in the structure. On the subject of
twinning - do you have the original diffraction images, and is there enough
data to re-process in a lower space group? Sometimes it takes only a few
non-symmetrical atoms to break an apparent higher symmetry, in which case
there may be issues with twin detection or lower non-apparent symmetry.

The amount of detail you provided isn't sufficient to draw any further
conclusions, besides trying lower symmetry.

Artem

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Cui
Sheng
Sent: Monday, June 02, 2008 5:24 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] gfp problem

Dear all:

We have an unrefinable protein complex structure with one of the component
is just a gfp. The space group shows as a P6122, a notorious space group for
twining. But I could not detect any sign of twinnig using various of
softwares. The R_free traps currently at 0.34 and no more space to impove.
We observed only one of the two gfps in ASU is badly fited to the density.
In this molecule, there are quite some loops in contact to solution looked
totally mesy and all the parts rest looks just fine. Take those mesy loops
in to the consideration,account for only 3% residues in ASU we are still
bitterly puzzled by the fact that our structure is 5% or more away from a
refined structure.

Native dataset collected at 2.5A
Molecular replacement gives unique solution using normal gfp as searching
model.

any good ideas solving our problem?

many thanx in advance

sheng

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CCP4bb <-- 2008 <-- June 2008 <-- 02 June 2008

Previous message:
Subject: gfp problem
From: Cui Sheng sheng {- at -} LMB {- dot -} UNI-MUENCHEN {- dot -} DE
Date: 2008-06-02

Next message:
Subject: Re: gfp problem
From: "Mark J {- dot -} van Raaij" vanraaij {- at -} USC {- dot -} ES
Date: 2008-06-03