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Re: [ccp4bb] Crystals grown directly on loop?
 

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CCP4bb <-- 2008 <-- June 2008 <-- 04 June 2008
Previous message:
Subject: Re: crystallisation and mosaicity
From: Paul Paukstelis paul {- at -} ICMB {- dot -} UTEXAS {- dot -} EDU
Date: 2008-06-03 		Next message:
Subject: BiFC, PCA, split-GFP for structure validation
From: Matthias Wilmanns matthias {- dot -} wilmanns {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2008-06-04


Subject: Re: Crystals grown directly on loop?
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-06-04
Dear Jacob and Mathew,

one night at the pub a few months ago a colleague and I
had been thinking along similar lines but we were rather envisaging
microcapillaries feeding the mother liquor components into the loop at
controlled rates from the stem ... expensive hi-tech loops to be sure
but maybe not so in a few years should technology stay with us for
longer. Time will tell.

Good luck with your efforts!

Pietro
> Dear Jacob,
>
> We have been working on this for an year or so. We have a paper partially in the review process. Unfortunately, the referees were not very excited about the idea. However, we are developing some automation to speed up the time consuming process of placing the drops on the loops.
>
> This method seems to have some advantges and some issues in using the known crystallization conditions. This could also give trouble with solutions containing volatile compounds.
>
> Kind regards,
> Mathews
>
>
> -----Original Message-----
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller
> Sent: Tuesday, June 03, 2008 1:14 PM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] Crystals grown directly on loop?
>
> Dear crystallographers,
>
> Has anyone ever tried to grow crystals directly on some kind of mountable support, such as some kind of loop or film, which could be frozen directly?
> I understand that there are some microfluidic plates through which the crystals can be diffraction-screened, but what about for more conventional, known crystallization conditions? It seems that this would be a spectacular way to decrease damages/stresses caused by handling...
>
> Jacob Keller
>
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> Dallos Laboratory
> F. Searle 1-240
> 2240 Campus Drive
> Evanston IL 60208
> lab: 847.491.2438
> cel: 773.608.9185
> email: j-keller2@northwestern.edu
> *******************************************
--
Pietro Roversi
Sir William Dunn School of Pathology, Oxford University
South Parks Road, Oxford OX1 3RE, England UK
Tel. 0044-1865-275385

CCP4bb navigation

CCP4bb <-- 2008 <-- June 2008 <-- 04 June 2008
Previous message:
Subject: Re: crystallisation and mosaicity
From: Paul Paukstelis paul {- at -} ICMB {- dot -} UTEXAS {- dot -} EDU
Date: 2008-06-03 		Next message:
Subject: BiFC, PCA, split-GFP for structure validation
From: Matthias Wilmanns matthias {- dot -} wilmanns {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2008-06-04


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