[ccp4bb] BiFC, PCA, split-GFP for structure validation

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystals grown directly on loop?
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-06-04

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Subject: mmdb vs. gcc4.3
From: "Debreczeni, Judit" Judit {- dot -} Debreczeni {- at -} ASTRAZENECA {- dot -} COM
Date: 2008-06-04

Subject: BiFC, PCA, split-GFP for structure validation
From: Matthias Wilmanns matthias {- dot -} wilmanns {- at -} EMBL-HAMBURG {- dot -} DE
Date: 2008-06-04

Hi,

I have been looking for examples in the literature for an article on
the use of bimolecular fluorescence complementation (BiFC), protein-
fragment complementation (PCA), split-GFP and variations (different
names for basically the same method) for validation of protein
assemblies or verification of structure-based hypotheses.

In case you have been using BIFC/PCA/split-GFP for validation of your
own structure(s) or verification of hypotheses using structural
models I am kindly asking to send me a brief email (wilmanns(ad)embl-
hamburg.de), quoting a reference where the work has been published.

Thank you,
Matthias Wilmanns, EMBL-Hamburg.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Crystals grown directly on loop?
From: Pietro Roversi pietro {- dot -} roversi {- at -} PATH {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-06-04

Next message:
Subject: mmdb vs. gcc4.3
From: "Debreczeni, Judit" Judit {- dot -} Debreczeni {- at -} ASTRAZENECA {- dot -} COM
Date: 2008-06-04