Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

[ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity
 

Basic tutorials: 		 
 

CCP4bb navigation

CCP4bb <-- 2008 <-- June 2008 <-- 05 June 2008
Previous message:
Subject: Re: crystallisation and mosaicity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-06-05 		Next message:
Subject: Re: 3D model building without CRT monitor
From: Vangelis Christodoulou e {- dot -} christodoulou {- at -} NKI {- dot -} NL
Date: 2008-06-05


Subject: Fwd: crystallisation and mosaicity
From: Patrick Loll ploll {- at -} DREXELMED {- dot -} EDU
Date: 2008-06-05

>
> Hi Charlie,
>
> yes you are right, but I assumed if people see a cloud of condensed
> fog over their LN2 bath they should remove that by
> a) filling up the bowl completely e.g. some LN2 drips out of the bowl
> b) blow the fog away before you dip

True; this has been demonstrated quite rigorously:

A general method for hyperquenching protein crystals
Journal Journal of Structural and Functional Genomics
Volume 8, Number 4 / December, 2007
DOI 10.1007/s10969-007-9029-0
Pages 141-144

AND

J. Appl. Cryst. (2006). 39, 805-811 [ doi:10.1107/S0021889806037484 ]
Hyperquenching for protein cryocrystallography
M. Warkentin, V. Berejnov, N. S. Husseini and R. E. Thorne


>

------------------------------------------------------------------------
---------------
Patrick J. Loll, Ph. D.
Professor of Biochemistry & Molecular Biology
Director, Biochemistry Graduate Program
Drexel University College of Medicine
Room 10-102 New College Building
245 N. 15th St., Mailstop 497
Philadelphia, PA 19102-1192 USA

(215) 762-7706
pat.loll@drexelmed.edu


CCP4bb navigation

CCP4bb <-- 2008 <-- June 2008 <-- 05 June 2008
Previous message:
Subject: Re: crystallisation and mosaicity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-06-05 		Next message:
Subject: Re: 3D model building without CRT monitor
From: Vangelis Christodoulou e {- dot -} christodoulou {- at -} NKI {- dot -} NL
Date: 2008-06-05


ProteinCrystallography.org: Copyright 2006-2008 by Quid United Ltd