Re: [ccp4bb] Fwd: [ccp4bb] crystallisation and mosaicity

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: 3D model building without CRT monitor
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-06-05

Next message:
Subject: Re: 3D model building without CRT monitor
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-06-05

Subject: Re: Fwd: crystallisation and mosaicity
From: Petr Leiman leiman {- at -} PURDUE {- dot -} EDU
Date: 2008-06-05

>> yes you are right, but I assumed if people see a cloud of condensed
>> fog over their LN2 bath they should remove that by
>> a) filling up the bowl completely e.g. some LN2 drips out of the bowl
>> b) blow the fog away before you dip

I think the original poster meant the relatively low heat conduction of
liquid N2, which causes boiling around the crystal immediately after
plunging.

The best way to freeze things is to put a small container of liquid ethane
or propane into a liquid N2 bowl, and plunge into the ethane/propane (this
methods was suggested earlier).

Petr

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: 3D model building without CRT monitor
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-06-05

Next message:
Subject: Re: 3D model building without CRT monitor
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-06-05