Re: [ccp4bb] cryo-cooling, was: Re: [ccp4bb] crystallisation and mosaicity

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: cryo-cooling, was: Re: crystallisation and mosaicity
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2008-06-06

Next message:
Subject: Re: cryo-cooling
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-06-07

Subject: Re: cryo-cooling, was: Re: crystallisation and mosaicity
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-06-06

>
>
> But for some crystals flash-cooling is better at temperatures higher
> than in the 77 K to 100 K regime for unknown reasons. This can be one
> reason why flash-cooling in the gas stream occassionally is better.
> It is also why liquid propane worked for Raji E. and Karolin L.: they
> were using a temperature above the freezing point of propane.
>
Hi Jim,

could the reason for this be cold annealing ? You have an imperfect
crystal lattice which thanks to the slow cooling can adjust it's lattice
slightly to yield better diffracting crystals ? In my simple mind this
is how I imagine flash annealing procedures.

Juergen

--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: cryo-cooling, was: Re: crystallisation and mosaicity
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2008-06-06

Next message:
Subject: Re: cryo-cooling
From: Kay Diederichs kay {- dot -} diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-06-07