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Re: [ccp4bb] Unusual Difference Fourier near Methionines
 

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CCP4bb <-- 2007 <-- April 2007 <-- 13 April 2007
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Subject: Unusual Difference Fourier near Methionines
From: Ethayathulla Abdulsamath ethaya_aiims {- at -} REDIFFMAIL {- dot -} COM
Date: 2007-04-13 		Next message:
Subject: Re: Additive screens
From: Erika Mancini erika {- at -} STRUBI {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-04-13


Subject: Re: Unusual Difference Fourier near Methionines
From: David Schwefel rock34 {- at -} GMX {- dot -} NET
Date: 2007-04-13
When I experienced strong difference density near methionines, It could be explained by an alternative conformation of the residue...

greetings, david


-------- Original-Nachricht --------
Datum: Fri, 13 Apr 2007 09:51:09 -0000
Von: Ethayathulla Abdulsamath
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Unusual Difference Fourier near Methionines

>
> Dear all
>
> I am doing one structure at 2.6A resolution where I found unusual density
> near methionines. Actually three methionines come close together nearby and
> I get difference even at 5sigma cutoff. I don't understand nature of the
> density. The amino acid sequnce is same I mean met by chemical sequncing so
> there is no change amino acid residues. I am sending the snapshot of the
> difference fourier observed at methionines. The crystal belong to R32 system
> with one molecule and biological trimer. In snapshot two of the methionines
> are symmetry related.
>
> Details of the data collection.
> Data collected at synchrotron
>
> Overall Rsym = 6 % and I/sigma = 2.1. Completeness = 99%
>
> Can anyone suggestion what could be chemical nature for the difference
> fourier.
>
> Thanks in advance
>
> Ethayathulla
>
> ###################################################
> A.S.Ethayathulla,Ph.D.
> Department of Biophysics
> All India Institute of Medical Sciences
> Ansari Nagar
> New Delhi-110029
> India.
> ###################################################

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