Re: [ccp4bb] inclusion bodies

- Protein crystallography

Main steps:

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: query on DNA-protein complex preparation for crystallization
From: Phoebe Rice price {- at -} UCHICAGO {- dot -} EDU
Date: 2008-06-24

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Subject: Re: Econo column with AKTA FPLC
From: Nathaniel Echols nathaniel {- dot -} echols {- at -} GMAIL {- dot -} COM
Date: 2008-06-24

Subject: Re: inclusion bodies
From: Arundhati Chattopadhyay arundhat {- at -} GMAIL {- dot -} COM
Date: 2008-06-24

You can coexpress the enzyme with chaperone (e.g GroEL and GroES)....very
often this is very successful. (There are bunches of ways though...)

Best wishes,

Arundhati

2008/6/23 yangliuqing :

> Hello,everyone!
> Sorry to bother you!
> Now I have an enzyme cloned in pQE2 ,expressed in E.Coli M15 at room
> temperature,but there are a lot of inclusion bodies,perhaps 90%,it is a big
> trouble because I need to get much enzyme.
> Is there anyone knows how to solve?
> Thank you very much!
> Best wishes!
> liuqing
>
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: query on DNA-protein complex preparation for crystallization
From: Phoebe Rice price {- at -} UCHICAGO {- dot -} EDU
Date: 2008-06-24

Next message:
Subject: Re: Econo column with AKTA FPLC
From: Nathaniel Echols nathaniel {- dot -} echols {- at -} GMAIL {- dot -} COM
Date: 2008-06-24