Re: [ccp4bb] pH gradient in Mono Q

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: pH gradient in Mono Q
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-06-25

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Subject: Re: pH gradient in Mono Q
From: Matthew Chu linghonmatthew {- at -} GMAIL {- dot -} COM
Date: 2008-06-25

Thanks Guenter and Andreas,

Yea, I have taken a look for the Mono P before, I thought the material they
used in Mono P is basically the same as in Mono Q and I found the
book"Protein Purification Protocols: Second Edition" by
Paul Cutler mentioned that phosphate buffer can also generate a continuous
gradient....that's why I reckon we can also perform pH gradient in Mono Q.
But I am worrying if it is a good idea to go for it, as both mono Q or mono
P are quite expensive.

Matt

2008/6/24 Andreas Förster :

> Hey Matt,
>
> it seems to me that what you're asking for is chromatofocusing. See the
> official GE documentation:
>
> http://www6.gelifesciences.com/aptrix/upp00919.nsf/Content/WD:Chromatofocusin(260949098-R350)
>
> The proprietary buffers are a bit expensive, but as you found out, they're
> a bit complicated to make. I don't know if you'd ruin your Mono Q with a pH
> gradient. If in doubt, buy one of the dedicated Mono P column.
>
> Hope that helps.
>
>
> Andreas
>
>
> Matthew Chu wrote:
>
>> Dear All,
>>
>> Sorry for off-topic question. Does anyone have any experience in purifying
>> protein using pH gradient in Mono Q column?
>>
>> I have been googling for a whole day, only one paper was found to mention
>> performing pH gradient in Mono Q, but in a mixture of amine buffering
>> species, which is a bit too complicated (J. Chromatogr. A 1164 (2007) 181 -
>> 188. Can Tris-Cl/Tris-base or phosphate buffer give a linear pH gradient
>> from pH 8.0 to 4.0? Is it usual to perform pH gradient in Mono Q as I don't
>> want to ruin my Mono Q column...
>>
>> Any suggestions are welcome. Thanks in advance!
>>
>> Kind regards,
>> Matt
>>
>>
>> ----------------------------------------------------------------------------
>> Matthew LH Chu
>> PhD Student
>> School of Pharmacy and Pharmaceutical Sciences
>> University of Manchester
>>
>> ----------------------------------------------------------------------------
>>
>

--
----------------------------------------------------------------------------
Matthew LH Chu
PhD Student
School of Pharmacy and Pharmaceutical Sciences
University of Manchester
----------------------------------------------------------------------------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: pH gradient in Mono Q
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-06-25

Next message:
Subject: COURSE ANNOUNCEMENT - BIOCRYS2008 - REMINDER
From: Daniele de Sanctis de {- dot -} sanctisd {- at -} GMAIL {- dot -} COM
Date: 2008-06-25