Re: [ccp4bb] cell line

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problem about Chainsaw
From: Xiang Liu pku06a15 {- at -} YAHOO {- dot -} COM {- dot -} CN
Date: 2008-06-25

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Subject: cell line
From: Exec shettylv {- at -} GMAIL {- dot -} COM
Date: 2008-06-25

Subject: Re: cell line
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-06-25

Highly recommended:

Animal Cell Culture: A Practical Approach
by R. Ian Freshney (Editor)

Artem
-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Exec
Sent: Tuesday, June 24, 2008 10:59 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cell line

Hi everyone,

can anyone tell me the protocol to revive human corneal cell lineor any
cell line which requires serum free media

i usually used serum based media initially.....later switched it to serum
free media for subculturing....only in case of human corneal cell line
with the help of flask coated with fibronectin, BSA, and
collagen..........but recently i m finding difficult to revive it....it
deattaches after 1 or 2 days..what might be the reason? also we are unable
to revive pRSV-T cell line using flask coating method. can anyone help on
the same.

thanks

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: problem about Chainsaw
From: Xiang Liu pku06a15 {- at -} YAHOO {- dot -} COM {- dot -} CN
Date: 2008-06-25

Next message:
Subject: cell line
From: Exec shettylv {- at -} GMAIL {- dot -} COM
Date: 2008-06-25