[ccp4bb] salt or protein?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Differentiating bound Mn & Ca.
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-16

Next message:
Subject: Re: Differentiating bound Mn & Ca.
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2007-04-16

Subject: salt or protein?
From: Sreeram Mahesh mahesh {- at -} PHYSICS {- dot -} IISC {- dot -} ERNET {- dot -} IN
Date: 2007-04-16

Hi All!

I have been trying to screen for my protein crystals, from the
crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate
Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6.
Two different kinds of crystals observed: rod shaped and thin platy
ones. Whenever I am trying to collect data from the rod shaped ones I am
getting dominantly salt patterns but also spots at 15 A resolution bin
repeatedly, where some of the spots very much look like from protein!
what can be the reason for this? if anyone has come across similar
situation please help.

with regards,
Sreeram Mahesh

Research Student
Prof S Ramakumar's lab
PHYSICS department
IISc Bangalore-560 012.
ph:080-2293 2718.
mobile: 9241145183.

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Differentiating bound Mn & Ca.
From: Eleanor Dodson ccp4 {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-04-16

Next message:
Subject: Re: Differentiating bound Mn & Ca.
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2007-04-16