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[ccp4bb] salt or protein? |
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CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 16 April 2007Subject: salt or protein? From: Sreeram Mahesh mahesh {- at -} PHYSICS {- dot -} IISC {- dot -} ERNET {- dot -} IN Date: 2007-04-16 I have been trying to screen for my protein crystals, from the crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6. Two different kinds of crystals observed: rod shaped and thin platy ones. Whenever I am trying to collect data from the rod shaped ones I am getting dominantly salt patterns but also spots at 15 A resolution bin repeatedly, where some of the spots very much look like from protein! what can be the reason for this? if anyone has come across similar situation please help. with regards, Sreeram Mahesh Research Student Prof S Ramakumar's lab PHYSICS department IISc Bangalore-560 012. ph:080-2293 2718. mobile: 9241145183. -- This message has been scanned for viruses and dangerous content by MailScanner, and is believed to be clean. CCP4bb navigationCCP4bb <-- 2007 <-- April 2007 <-- 16 April 2007 |
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