[ccp4bb] Concentrating protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Friedel vs Bijvoet
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2008-06-27

Next message:
Subject: Re: Concentrating protein
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-06-27

Subject: Concentrating protein
From: Exec shettylv {- at -} GMAIL {- dot -} COM
Date: 2008-06-27

Dear All,

we have GCSF protein produced in inclusion bodies. we solubilise it refold
it and then concentrate it using proflux system. still the concentration
of the protein we get is less and volume is more for us to load in Ion
exchange chromatography. is there any simple technique that can be
performed in lab without using any hi-fi instrument to concentrate the
protein in small volume of buffer. the protein we obtain is about 0.7
mg/ml and we get 450 ml solution. our column is 110ml lab scale and we
have to work in that only. i have heard of NH4SO4 precipitation. but it
requires protein conc more than 1 mg/ml.

kindly help me to progress in my experiment.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Friedel vs Bijvoet
From: Bernhard Rupp bernhardrupp {- at -} SBCGLOBAL {- dot -} NET
Date: 2008-06-27

Next message:
Subject: Re: Concentrating protein
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-06-27