Re: [ccp4bb] Differentiating bound Mn & Ca.

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Subject: Re: Differentiating bound Mn & Ca.
From: "Miller, Mitchell D {- dot -} " mmiller {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-16

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Date: 2007-04-16

Subject: Re: Differentiating bound Mn & Ca.
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-16

Dave,

Others have given many excellent suggestions :) I'd like to mention that
to a protein biochemist such as myself, another obvious solution would be
to take an aliquot of the protein for a metal analysis (via ICP or AA).
ICP is more sensitive. Both Mn and Ca will light up the spectrum like new
year's tree lights.

Artem

> The obvious solution is go to a synchrotron and scan around the Mn edge
> and
> see what happens, however, whilst waiting for beam time, is there any way

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CCP4bb <-- 2007 <-- April 2007 <-- 16 April 2007

Previous message:
Subject: Re: Differentiating bound Mn & Ca.
From: "Miller, Mitchell D {- dot -} " mmiller {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2007-04-16

Next message:
Subject: Re: Selenomethionine reduction v.s. disulfide bond
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-04-16