Re: [ccp4bb] salt or protein?

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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From: Randy Read rjr27 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2007-04-16

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Date: 2007-04-16

Subject: Re: salt or protein?
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-04-16

Hi Sreeram,

assuming you have plenty of those crystals, why don't you loop a few
pass them through a drop of your reservoir for washing and load them on
a SDS gel ?

Juergen

Sreeram Mahesh wrote:

> Hi All!
>
> I have been trying to screen for my protein crystals, from the
> crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate
> Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6.
> Two different kinds of crystals observed: rod shaped and thin platy
> ones. Whenever I am trying to collect data from the rod shaped ones I
> am getting dominantly salt patterns but also spots at 15 A resolution
> bin repeatedly, where some of the spots very much look like from protein!
> what can be the reason for this? if anyone has come across similar
> situation please help.
>
> with regards,
> Sreeram Mahesh
>
>
> Research Student
> Prof S Ramakumar's lab
> PHYSICS department
> IISc Bangalore-560 012.
> ph:080-2293 2718.
> mobile: 9241145183.
>
>

--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Openings for Phaser team
From: Randy Read rjr27 {- at -} CAM {- dot -} AC {- dot -} UK
Date: 2007-04-16

Next message:
Subject: Postdoctoral position in structural immunology
From: "Dirk M {- dot -} Zajonc" dzajonc {- at -} LIAI {- dot -} ORG
Date: 2007-04-16