[ccp4bb] RE : [ccp4bb] Disordered domains in crystal strutures

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: enforce planarity in user defined isopeptide link?
From: Sarah Hymowitz hymowitz {- dot -} sarah {- at -} GENE {- dot -} COM
Date: 2008-07-01

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From: "Jens T {- dot -} Kaiser" kaiser {- at -} CALTECH {- dot -} EDU
Date: 2008-07-01

Subject: RE : Disordered domains in crystal strutures
From: Stephane Mouilleron Stephane {- dot -} Mouilleron {- at -} CANCER {- dot -} ORG {- dot -} UK
Date: 2008-07-01

Dear Gina,

We have recently solved a structure of the glucosamine 6P synthase from E.coli (GlmS) and surpringsly, although the whole protein was present in this crystal form, no electron density was observed for the glutaminase domain (240 residues out of 600), indicating its mobility.

Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B.
Ordering of C-terminal loop and glutaminase domains of glucosamine-6-phosphate synthase promotes sugar ring opening and formation of the ammonia channel.
J Mol Biol. 2008 Apr 4;377(4):1174-85. Epub 2008 Feb 4.
PMID: 18295797

This domain mobility is functionally essential for the catalysis. Although this domain is mobile in the absence of ligand, it is ordered in two previous structures solved in an “active” and “inactive” conformation:

- In the “inactive” conformation, its glutaminase domain was resolved in the density but the density was very poor. Analysis of the TLS parameters shown large anisotropic displacements.

- In the “active” conformation with its glutaminase domain well ordered but in a different conformation (21 degrees rotation).

Mouilleron S, Golinelli-Pimpaneau B.
Domain motions of glucosamine-6P synthase: comparison of the anisotropic displacements in the crystals and the catalytic hinge-bending rotation.
Protein Sci. 2007 Mar;16(3):485-93.
PMID: 17322533

Mouilleron S, Badet-Denisot MA, Golinelli-Pimpaneau B.
Glutamine binding opens the ammonia channel and activates glucosamine-6P synthase.
J Biol Chem. 2006 Feb 17;281(7):4404-12. Epub 2005 Dec 9.
PMID: 16339762

I hope it will help you,

Cheers

Stephane

-------- Message d'origine--------
De: CCP4 bulletin board de la part de Gina Clayton
Date: sam. 28/06/2008 23:30
À: CCP4BB@JISCMAIL.AC.UK
Objet : [ccp4bb] Disordered domains in crystal strutures

Dear CCP4ers

can anyone recommend papers describing crystal structures of proteins
with a large functionally important disordered domain or domains.

Thanks in advance

Gina

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: enforce planarity in user defined isopeptide link?
From: Sarah Hymowitz hymowitz {- dot -} sarah {- at -} GENE {- dot -} COM
Date: 2008-07-01

Next message:
Subject: Re: Weakest protein-protein complex crystallised
From: "Jens T {- dot -} Kaiser" kaiser {- at -} CALTECH {- dot -} EDU
Date: 2008-07-01