[ccp4bb] needles not improved

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Weakest protein-protein complex crystallised
From: "John A {- dot -} Newitt" newittja {- at -} COMPUSERVE {- dot -} COM
Date: 2008-07-01

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Subject: Re: needles not improved
From: Janet Newman Janet {- dot -} Newman {- at -} CSIRO {- dot -} AU
Date: 2008-07-01

Subject: needles not improved
From: Marta_Martínez mmartine {- at -} UNIZAR {- dot -} ES
Date: 2008-07-01

Hi

Anyone can help me?.

I have crystals that are needles (with twinning, apparently). The
crystalization condition is PEG 4K, 0.1 M NaAc pH 4.6 and 0.2 M ammonium
acetate. I have tried in this condition with detergents and additives and
with ligands but nothing improves the needles. Always needles. Are there
any other stategies to try?.
Thank you

Marta

e-mail: mmartine@unizar.es

>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Weakest protein-protein complex crystallised
From: "John A {- dot -} Newitt" newittja {- at -} COMPUSERVE {- dot -} COM
Date: 2008-07-01

Next message:
Subject: Re: needles not improved
From: Janet Newman Janet {- dot -} Newman {- at -} CSIRO {- dot -} AU
Date: 2008-07-01