Re: [ccp4bb] needles not improved

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: needles not improved
From: Marta_Martínez mmartine {- at -} UNIZAR {- dot -} ES
Date: 2008-07-01

Next message:
Subject: Re: Weakest protein-protein complex crystallised
From: Philippe DUMAS p {- dot -} dumas {- at -} IBMC {- dot -} U-STRASBG {- dot -} FR
Date: 2008-07-01

Subject: Re: needles not improved
From: Janet Newman Janet {- dot -} Newman {- at -} CSIRO {- dot -} AU
Date: 2008-07-01

You could try the matrix seeding method, popularised by Allan D'Arcy et al (Acta Cryst. (2007). D63, 550-554)

Crush up your needles, and use them to seed into a new round of screening. You may be able to jump into a new crystal system this way

Janet

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Marta Martínez
Sent: Tuesday, 1 July 2008 5:03 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] needles not improved

Hi

Anyone can help me?.

I have crystals that are needles (with twinning, apparently). The
crystalization condition is PEG 4K, 0.1 M NaAc pH 4.6 and 0.2 M ammonium
acetate. I have tried in this condition with detergents and additives and
with ligands but nothing improves the needles. Always needles. Are there
any other stategies to try?.
Thank you

Marta

e-mail: mmartine@unizar.es

>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: needles not improved
From: Marta_Martínez mmartine {- at -} UNIZAR {- dot -} ES
Date: 2008-07-01

Next message:
Subject: Re: Weakest protein-protein complex crystallised
From: Philippe DUMAS p {- dot -} dumas {- at -} IBMC {- dot -} U-STRASBG {- dot -} FR
Date: 2008-07-01