[ccp4bb] Hybrid enzymes

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Weakest protein-protein complex crystallised
From: Philippe DUMAS p {- dot -} dumas {- at -} IBMC {- dot -} U-STRASBG {- dot -} FR
Date: 2008-07-01

Next message:
Subject: Sequence of crystallised protein fragment
From: Klaus Futterer k {- dot -} futterer {- at -} BHAM {- dot -} AC {- dot -} UK
Date: 2008-07-01

Subject: Hybrid enzymes
From: Narayanan Ramasubbu ramasun1 {- at -} UMDNJ {- dot -} EDU
Date: 2008-07-01

Dear All:
I am working with two enzymes (40 kDa each). The crystal structures of
the two enzymes are known.
I have made a hybrid enzyme of the two individual ones.
This chimeric/hybrid enzyme is soluble and exhibits activity of the two
individual enzymes.
My questions are as follows:
1. Could I use one of the enzyme's structure to solve the crystal
structure of the hybrid by MR?
2. Has anyone working with such hybrids (?) noticed any increase in
specific activity of either of the enzymes?
Interestingly, the two enzymes work on the same substrate polymer. One
is a hydrolase another is an esterase.

Thanks a lot.
Subbu

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Weakest protein-protein complex crystallised
From: Philippe DUMAS p {- dot -} dumas {- at -} IBMC {- dot -} U-STRASBG {- dot -} FR
Date: 2008-07-01

Next message:
Subject: Sequence of crystallised protein fragment
From: Klaus Futterer k {- dot -} futterer {- at -} BHAM {- dot -} AC {- dot -} UK
Date: 2008-07-01