[ccp4bb] Sequence of crystallised protein fragment

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Hybrid enzymes
From: Narayanan Ramasubbu ramasun1 {- at -} UMDNJ {- dot -} EDU
Date: 2008-07-01

Next message:
Subject: Re: Hybrid enzymes
From: fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT
Date: 2008-07-01

Subject: Sequence of crystallised protein fragment
From: Klaus Futterer k {- dot -} futterer {- at -} BHAM {- dot -} AC {- dot -} UK
Date: 2008-07-01

We have a 150 kDa protein that reproducibly crystallises at one of
the Hampton Screen conditions. However, we know from SDS gel analysis
that the crystals contain only a 45 kDa fragment, that forms through
proteolytic cleavage over time. We would like to determine the
sequence boundaries of the fragment.

We believe a combination of N-terminal sequencing plus MS analysis
might give us the information we need, but I was wondering whether
the ccp4bb community has other suggestions.

Klaus

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Klaus Fütterer, Ph.D.

School of Biosciences P: +44-(0)-121-414 5895
University of Birmingham F: +44-(0)-121-414 5925
Edgbaston E: k.futterer@bham.ac.uk
Birmingham, B15 2TT, UK W: www.biochemistry.bham.ac.uk/klaus/
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Hybrid enzymes
From: Narayanan Ramasubbu ramasun1 {- at -} UMDNJ {- dot -} EDU
Date: 2008-07-01

Next message:
Subject: Re: Hybrid enzymes
From: fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT
Date: 2008-07-01