Re: [ccp4bb] Sequence of crystallised protein fragment

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

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   - Amino Acids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Sequence of crystallised protein fragment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-07-02

Subject: Re: Sequence of crystallised protein fragment
From: Matthew Chu linghonmatthew {- at -} GMAIL {- dot -} COM
Date: 2008-07-02

N-terminal sequencing / MS for intact mass analysis are the only ways that I
can think of.....

Matt

2008/7/1 Klaus Futterer :

> We have a 150 kDa protein that reproducibly crystallises at one of the
> Hampton Screen conditions. However, we know from SDS gel analysis that the
> crystals contain only a 45 kDa fragment, that forms through proteolytic
> cleavage over time. We would like to determine the sequence boundaries of
> the fragment.
>
> We believe a combination of N-terminal sequencing plus MS analysis might
> give us the information we need, but I was wondering whether the ccp4bb
> community has other suggestions.
>
> Klaus
>
>
>
>
>
>
> ---------------------------------------------------------------------
>
> Klaus Fütterer, Ph.D.
>
> School of Biosciences P: +44-(0)-121-414 5895
> University of Birmingham F: +44-(0)-121-414 5925
> Edgbaston E: k.futterer@bham.ac.uk
> Birmingham, B15 2TT, UK W: www.biochemistry.bham.ac.uk/klaus/
> ---------------------------------------------------------------------
>

----------------------------------------------------------------------------
Matthew LH Chu
PhD Student
School of Pharmacy and Pharmaceutical Sciences
University of Manchester
----------------------------------------------------------------------------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Q210 detector for sale!
From: James Holton JMHolton {- at -} LBL {- dot -} GOV
Date: 2008-07-01

Next message:
Subject: Re: Sequence of crystallised protein fragment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-07-02