Re: [ccp4bb] Sequence of crystallised protein fragment

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Sequence of crystallised protein fragment
From: Matthew Chu linghonmatthew {- at -} GMAIL {- dot -} COM
Date: 2008-07-02

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Subject: Re: Sequence of crystallised protein fragment
From: Kornelius Zeth kornelius {- dot -} zeth {- at -} TUEBINGEN {- dot -} MPG {- dot -} DE
Date: 2008-07-02

ESI-MS at a precision of +-2 Da should work alone.

Best wishes

Kornelius

On Tue, 1 Jul 2008 19:32:56 +0100
Matthew Chu wrote:
> N-terminal sequencing / MS for intact mass analysis are the only ways that I
> can think of.....
>
> Matt
>
> 2008/7/1 Klaus Futterer :
>
> > We have a 150 kDa protein that reproducibly crystallises at one of the
> > Hampton Screen conditions. However, we know from SDS gel analysis that the
> > crystals contain only a 45 kDa fragment, that forms through proteolytic
> > cleavage over time. We would like to determine the sequence boundaries of
> > the fragment.
> >
> > We believe a combination of N-terminal sequencing plus MS analysis might
> > give us the information we need, but I was wondering whether the ccp4bb
> > community has other suggestions.
> >
> > Klaus
> >
> >
> >
> >
> >
> >
> > ---------------------------------------------------------------------
> >
> > Klaus Fütterer, Ph.D.
> >
> > School of Biosciences P: +44-(0)-121-414 5895
> > University of Birmingham F: +44-(0)-121-414 5925
> > Edgbaston E: k.futterer@bham.ac.uk
> > Birmingham, B15 2TT, UK W: www.biochemistry.bham.ac.uk/klaus/
> > ---------------------------------------------------------------------
> >
>
>
>
>
> ----------------------------------------------------------------------------
> Matthew LH Chu
> PhD Student
> School of Pharmacy and Pharmaceutical Sciences
> University of Manchester
> ----------------------------------------------------------------------------

----------------------------------------------
Kornelius Zeth
Max Planck Institute for Developmental Biology
Dept. Protein Evolution
Spemannstr. 35
72076 Tuebingen, Germany
kornelius.zeth@tuebingen.mpg.de
Tel -49 7071 601 323
Fax -49 7071 601 349

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Sequence of crystallised protein fragment
From: Matthew Chu linghonmatthew {- at -} GMAIL {- dot -} COM
Date: 2008-07-02

Next message:
Subject: CNS error message with MR
From: J Knight jtknight {- at -} MAIL {- dot -} UTEXAS {- dot -} EDU
Date: 2008-07-02