[ccp4bb] crystallization tips for acidic proteins

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Sequence of crystallised protein fragment
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-02

Next message:
Subject: Postdoctoral job in the laboratory of Michael Rossmann
From: Petr Leiman leiman {- at -} PURDUE {- dot -} EDU
Date: 2008-07-02

Subject: crystallization tips for acidic proteins
From: "Karakas, Erkan" karakas {- at -} CSHL {- dot -} EDU
Date: 2008-07-02

Hi,

I am working on an acidic protein with a pI value around 4.5. Although I can obtain proteins with decent solubility, I haven't been able obtain crystals. I was wondering if anyone would have some suggestions to increase the chances for crystallization.

Thanks,
Erkan

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Sequence of crystallised protein fragment
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-07-02

Next message:
Subject: Postdoctoral job in the laboratory of Michael Rossmann
From: Petr Leiman leiman {- at -} PURDUE {- dot -} EDU
Date: 2008-07-02