[ccp4bb] phosphorylation of protein is troublesome or not for crystallization

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CCP4bb <-- 2007 <-- April 2007 <-- 16 April 2007

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Subject: Crystallization Course: focus on membrane proteins 2007
From: "Stojanoff, Vivian" stojanof {- at -} BNL {- dot -} GOV
Date: 2007-04-16

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Subject: Re: phosphorylation of protein is troublesome or not for crystallization
From: Kurt Hoffmann hoffmann {- at -} MOLBIOTECH {- dot -} RWTH-AACHEN {- dot -} DE
Date: 2007-04-17

Subject: phosphorylation of protein is troublesome or not for crystallization
From: Rongjin Guan rjguan {- at -} GMAIL {- dot -} COM
Date: 2007-04-16

Dear all,

Below is a question my friend asked me, but I have never worked on phosphorylated proteins.
Has anyone worked on crystallizing phosphorylated proteins and can you comment on it?

Thanks

Rongjin Guan
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I would like to ask how feasible it is to crystalize protein containing post-translational modification, such as phosphorylation. To my limited knowledge, I think the heterogenicity of modified and unmodified proteins causes the major obstacle in the crystal structure. Is there any specific method to crystalized modified proteins (like phosphorylated proteins ) these days?

CCP4bb navigation

CCP4bb <-- 2007 <-- April 2007 <-- 16 April 2007

Previous message:
Subject: Crystallization Course: focus on membrane proteins 2007
From: "Stojanoff, Vivian" stojanof {- at -} BNL {- dot -} GOV
Date: 2007-04-16